Designing a P48-40 Chimeric Protein of Mycoplasma agalactiae and Highly Expression in E. coli, Applicable for Indirect ELISA
نویسندگان
چکیده
HIGHLIGHTS Chimeric protein P48-40 of Mycoplasma agalactiae was designed and expressed in E.coli BL21 (DE3). highly soluble form purified with yield 33mg/Lby using Nickel resin. Novel showed high performance for detection specific antibodies 100% sensitivity specificity.
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2023
ISSN: ['1678-4324', '1516-8913']
DOI: https://doi.org/10.1590/1678-4324-2023220381